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CALLUS INDUCTION OF GERBERA EXPLANTS WITH DIFFERENT GROWTH REGULATOR COMPOSITIONS

paper-details
 
Author Name: S. Parvin, A.F. Ona, H. Ahmad, ASM Nahiyan and AFM Jamal Uddin
Research Area: Agricultural Science
Volume: 06
Issue: 01
Page No: 68-73
Emailed: 1
Total Downloads: 345
Country: Bangladesh
PDF View: Details



An experiment was accomplished at Advanced Seed Research and Biotech Center (ASRBC), ACI, Dhaka, Bangladesh during the period of September to October, 2014 to evaluate the callus induction of gerbera explants under different growth hormone. Five explants (E1, Flower bud; E2, Capitulum; E3, Leaves; E4, Peduncle; and E5, Petiole) and six growth regulator composition T0: 0 mg/l BAP + 0 mg /l NAA, T1: 1 mg/l BAP + 0.01 mg /l NAA, T2:2 mg/l BAP + 0.01 mg /l NAA, T3: 3 mg/l BAP + 0.01 mg /l NAA, T4:4 mg/l BAP + 0.01 mg /l NAA, T5:5 mg/l BAP + 0.01 mg /l NAA were used in this experiment. Individually explant Eand treatment T3 showed best performance in all the studied characteristics. In case of combined effect, T3E1 exhibited best performance overall except percent survival where T2E1 showed the best result (97.3). So, flower bud can be considered as the most suitable explant for further experimentation in protocol development.