IN VITRO REGENERATION AND RAPID MULTIPLICATION OF TUBEROSE

An experiment was conducted at Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from July 2011 to March 2012 for In Vitro regeneration of tuberose. Experiment consisted ten combinations of different concentrated KIN and IAA as supplemented with MS medium. Early shoot (10.5 days), leaf (14.6 days) and root (16.0 days) initiation were found from MS+0.5 mg/L IAA +1.0 mg/L KIN. Maximum number of leaves (2.99/plantlet), length of leaf (5.48 cm), number of roots (5.69/plantlet) and length of root (3.49 cm) were also found from MS+0.5 mg/L IAA +1.0 mg/L KIN.