SUBSEQUENT ADDITION OF SPIRULINA INOCULUM AND GROWTH NUTRIENT IN CULTURE MEDIA UNDER CLOSED SYSTEM SPIRULINA CULTIVATION

An experiment was conducted on the Rooftop, Faculty of agriculture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during February to April, 2019 to study the influence of subsequent application of spirulina inoculum and nutrient medium on growth and yield of spirulina. The experiment conducted with four treatments, viz., recycled water after harvest (T₀), addition of spirulina inoculum (T₁), addition of subsequent nutrient (T₂) and addition of both inoculum and nutrient (T₃) following completely randomized design (CRD) with four replications. Data on different growth and yield parameters showed significant variations among them. Subsequent application of both spirulina inoculum and nutrient (T₃) exhibit significant differences with other studied treatment and influenced to increase the yield (241.6%) over control. On the other, addition of subsequent nutrient media (T₂) revealed (147.0%) higher yield over T₀. Addition of inoculum had no impact on pH but application of nutrient medium influenced pH which ensured optimum growth and maximize the BCR (3.49). So, the effectiveness of all the treatment, addition of nutrient at each after harvest would be the effective for commercial spirulina production.